WEBINAR: Precise and fast spectral FLIM at video rates with a confocal

Date: Tuesday, June 26, 2018
Place: London 4:00 pm I Berlin 5:00 pm I Boston 11:00 am I San Diego 8:00 am
Speaker: Prof. Kees Jalink, Ph.D.
Registration: Register here.
More information: kopecky@pragolab.cz

Join our free webinar 
Precise and fast spectral FLIM at video rates with a confocal 

Förster (Fluorescence) Resonance Energy Transfer (FRET)  has become a powerful tool to study protein-protein interactions and signal transduction in living cells and  Fluorescence Lifetime Imaging (FLIM)  is a robust inherently quantitative microscopy technique that can used to get reliable FRET data. In confocal microscopy, FLIM is typically read out by Time-Correlated Single Photon Counting (TCSPC). This requires expensive add-on hardware and is very slow.

In this webinar Prof Kees Jalink will present his research results acquired with the  Leica SP8 FALCON  , the first truly integrated solution for FLIM, capable of delivering benchmark results at least 10 times faster than conventional systems. 
Says Prof. Jalink about his findings  : ‘’We scrutinized the performance of the  SP8 FALCON   and report how it performed when recording agonist-induced changes in concentration of second messengers such as cAMP and Ca2+ with sub-micrometer precision at high speed allowing detection of even very fast signaling events. We concluded that the  SP8-FALCON   is ideal for fast FLIM screening applications in both fixed- and live-cell formats’’. 

Prof. Kees Jalink, Netherlands Cancer Institute, Amsterdam , studied biology, biophysics and archeology at Leiden University, before moving to the NKI, Amsterdam to complete his PhD. Kees has a long history of collaborating and advising industry, in particular in microscopy and pharmacological screening. Kees loves new technology, inside and outside the lab, and spends every free hour building new setups and tinkering with electronics. 

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In this webinar, you will learn

• The power of FRET for measuring protein-protein interactions and signal transduction in living cells
• How FLIM is a robust quantitative technique, ideal for use in FRET
• How the Leica SP8 FALCON system delivers high speed FLIM data for both living and fixed cells

Example of the LAS X Navigator with Leica SP8 FALCON

Straightforward acquisition of complex samples. High resolution mouse embryo mosaic image of 722 tiles containing 190 Megapixels.  FLIM  data fitted with four characteristic fluorescence lifetimes, color coded. Acquisition: 1:23 h. Analysis: 1:00 h