WEBINAR: Precise and fast spectral FLIM at video rates with a confocal
Date:
Tuesday, June 26, 2018
Place:
London 4:00 pm I Berlin 5:00 pm I Boston 11:00 am I San Diego 8:00 am
Speaker:
Prof. Kees Jalink, Ph.D.
Registration:
Register here.
More information:
kopecky@pragolab.cz
![FIIINAl.png](http://www2.leica-microsystems.com/l/149501/2018-06-06/3thnxt/149501/52726/FIIINAl.png)
Join our free webinar
Precise and fast spectral FLIM at video rates with a confocal
Förster (Fluorescence) Resonance Energy Transfer (FRET) has become a powerful tool to study protein-protein interactions and signal transduction in living cells and Fluorescence Lifetime Imaging (FLIM) is a robust inherently quantitative microscopy technique that can used to get reliable FRET data. In confocal microscopy, FLIM is typically read out by Time-Correlated Single Photon Counting (TCSPC). This requires expensive add-on hardware and is very slow.
In this webinar Prof Kees Jalink will present his research results acquired with the
Leica SP8 FALCON
, the first truly integrated solution for FLIM, capable of delivering benchmark results at least 10 times faster than conventional systems.
Says Prof. Jalink about his findings
: ‘’We scrutinized the performance of the
SP8 FALCON
and report how it performed when recording agonist-induced changes in concentration of second messengers such as cAMP and Ca2+ with sub-micrometer precision at high speed allowing detection of even very fast signaling events. We concluded that the
SP8-FALCON
is ideal for fast FLIM screening applications in both fixed- and live-cell formats’’.
![Jalink_Ausschnitt_cmyk_Kopie__002_.png](http://www2.leica-microsystems.com/l/149501/2018-06-06/3thnxy/149501/52730/Jalink_Ausschnitt_cmyk_Kopie__002_.png)
Click the button below to reserve your place in this free webinar:
Register here!
In this webinar, you will learn
- The power of FRET for measuring protein-protein interactions and signal transduction in living cells
- How FLIM is a robust quantitative technique, ideal for use in FRET
- How the Leica SP8 FALCON system delivers high speed FLIM data for both living and fixed cells
Example of the LAS X Navigator with Leica SP8 FALCON
![csm_Mouse-Embryo-ph3_02_db1ffe81a9.jpg](https://www.leica-microsystems.com/typo3temp/_processed_/9/b/csm_Mouse-Embryo-ph3_02_db1ffe81a9.jpg)
Straightforward acquisition of complex samples. High resolution mouse embryo mosaic image of 722 tiles containing 190 Megapixels. FLIM data fitted with four characteristic fluorescence lifetimes, color coded. Acquisition: 1:23 h. Analysis: 1:00 h